NuclearParticleDetector2D

API

The NuclearParticleDetector2D detects nuclei and particles inside these * nuclei and gives some statistics about particles, like particle number per * nuclei, particle size, sum of intensities, etc. Nuclei are detected via the * NucleusDetector2D by using the Niblack thresholding. As post-process the * nuclei channel is gamma corrected and filter with a small gaussian, due to * uneven illumination of the nuclei in the images. This may depends on the * input image data and can be switched off separately. Particles are detected * via the ParticleDetectorUWT2D.

see Nucleus Detector 2D

see Particle Detector UWT 2D

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Output:

• image with detected nuclei and particles

• *

  detected nuclei regions

• detected particle regions

  *

• table with statistic results

  
  

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Usage (standard view)

Required parameters:

• Nuclei Channel

  • Channel number of the * nuclei image channel

  • range [1, #ImageChannels]

  • *

    default: 2

• Particle * Channel

  • Channel number of the particle image channel

    *

  • range [1, #ImageChannels]

  • default: * 1

Supplemental parameters:

• Verbose

  • Output of additional messages * on console is disabled/enabled

  • default: * false

    
    

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Usage (advanced view):

Required parameters:

• Nuclei Channel

  • Channel number of the * nuclei image channel

  • range [1, #ImageChannels]

  • *

    default: 2

• Particle * Channel

  • Channel number of the particle image channel

    *

  • range [1, #ImageChannels]

  • default: * 1

• Use Gamma * Correction

  • Flag to use gamma correction for nuclei * channel

  • default: true

  *

• Gamma Value

  • Gamma correction value * for nuclei channel

  • range [0, 1]

  • default: * 0.5

• Use Gaussian * Filter

  • Flag to use gaussian filter for nuclei * channel

  • default: true

  *

• Nucleus Detector

  • Detector to use for * nuclei detection

  • Configurable via GUI (see Nucleus Detector 2D)

• Particle * Detector

  • Detector to use for particle detection

    *

  • Configurable via GUI (see Particle Detector UWT 2D)

Supplemental parameters:

• Verbose

  • Output of additional messages * on console is disabled/enabled

  • default: * false