This operator is for segmenting images or image sequences containing fluorescently labeled cells
Every frame is treated separately and the result is a binary image with the same dimensions as the input image
input image
image (sequence) to be segmented
channel
image channel to be segmented
minimum seed size
minimum size (number of pixels) of seed objects to be considered as cells
seed image
image that contains seeds (groups of pixels that don't have intensity value 0)
seeds are used as initialization for level set segmentation
if left empty, seeds are created automatically
median filter radius
radius (pixels) of median filter used for noise reduction
maximum number of iterations [advanced view]
maximum number of iterations for level set segmentation
γ [advanced view]
gamma correction with a value smaller than 1 is used to emphasize faintly fluorescing cells / cell parts
if background noise is very high the value should be increased
Verbose
output some additional information